Consumption of Yogurt Containing Probiotic Bifidobacterium Lactis Reduces Streptococcus mutans in Orthodontic Patients

Armelia Sari Widyarman, Shirley Trisna Yunita, Tjokro Prasetyadi


Background: Probiotic bacteria is commonly used as a food supplement intended to benefit the host by improving intestinal bacterial balance. Probiotics have also been investigated from the perspective of oral health. Objectives: The purpose of this study was to investigate the effect of daily intake of yogurt containing probiotic Bifidobacterium animalis subsp. lactis BB-12 (B. lactis) on salivary Streptococcus mutans (S. mutans) counts in patients undergoing fixed orthodontic treatment. Methods: Saliva samples were collected from each subject (n = 7; mean age, 21 years) using spitting method in centrifuge tubes at baseline and two weeks after daily probiotic yogurt consumption. B. lactis BB-12 and S. mutans ATCC 25175 were cultured in BHI-broth (37ºC, anaerobic conditions). After 48 hours incubation, the number of colonies on each dilution plate was used to extrapolate a standard curve. The total number of target DNA molecules were identified using Real-Time PCR followed by SYBR Green reagents and 16S rRNA gene specific primers S. mutans and B. lactis BB-12. Data were analyzed statistically using paired-sample t-tests. Results: Statistical evaluation indicated that there was a significant reduction in the presence of S. mutans before probiotic yogurt consumption, (4.73 ± 1.43) log10 CFU/mL and after two weeks of daily consumption of probiotic yogurt, (4.03 ± 0.77) log10 CFU/mL, p = 0.001. Moreover, no B. lactis was found in the saliva of any of the subjects before probiotic consumption, but after two weeks of consumption, B. lactis was found in the saliva of four subjects. Conclusions: Consuming probiotic yogurt containing B. lactis reduced the quantity of S. mutans in the saliva of subjects during fixed orthodontic treatment. Thus, the probiotic bacteria could be beneficial in improving oral health.

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Kour S, Verma VK, Sachan A, Singh K, Arora A, Kaur G. Role of probiotics in orthodontics. Rama Univ J Dent Sci. 2015;2(3):26-31.

Maret D, Marchal-Sixou C, Vergnes JN, Hamel O, Georgelin-Gurgel M, Van Der Sluis L, et al. Effect of fixed orthodontic appliances on salivary microbial parameters at 6 months: a controlled observational study. J Appl Oral Sci. 2014;22(1):38-43. DOI: 10.1590/1678-775720130318

Jung WS, Kim H, Park SY, Cho EJ, Ahn SJ. Quantitative analysis of changes in salivary mutans streptococci after orthodontic treatment Am J of Orthod Dentofacial Orthop. 2014;145(5):603-9. DOI: 10.1016/j.ajodo.2013.12.025

Widyarman AS, Widjaja SB, Idrus E. Strawberry extract’s effects on Enterococcus faecalis and Porphyromonas gingivalis biofilms in vitro. Sci Dent J. 2017;01:1-5. DOI:

Agrawal V, Kapoor S, Shah N. Role of ‘live microorganisms’ (probiotics) in prevention of caries: going on the natural way towards oral health. Indian J Multidiscip Dent. 2012; 2(3):491-496.

Gupta G. Probiotics and periodontal health. J Med Life. 2011;4(4): 387–394.

Haukioja A. Probiotics and oral health. Eur J Dent. 2010 Jul; 4(3): 348–355.

Singh RP, Damle SG, Chawla A. Salivary mutans streptococci and lactobacilli modulations in young children on consumption of probiotic ice-cream containing Bifidobacterium lactis BB-12 and Lactobacillus acidophillus La-5. Acta Odontol Scand. 2011;69(6):389-94. DOI: 10.3109/000/16357.2011.572289. Epub 2011 Apr 5.

Cagetti MG, Mastroberardino S, Milia E, Cocco F, Lingström P, Campus G. The use of probiotic strains in caries prevention: A systemic review. Nutrients. 2013;5(7):2530-50. Available from: DOI: 10.3390/nu5072530

Meurman JH, Stamatova I. Probiotics: contributions to oral health. Oral Dis. 2007;13(5):443-51. DOI: 10.1111/j.1601-0825.2007.01386.x

Vlieger AM, Schuren F, Benninga MA, Biesebeke R. Change in microbiota after consumption of Bifidobacterium lactis BB12 in combination with Lactobacillus paracasei CRL431 by healthy term infants In: Vlieger AM. editor. Complementary therapies in peadiatric gastroenterology: prevalence, safety and efficacy studies. Amsterdam: UvA-DARE-University of Amsterdam. 2009. 164-74. Available from:

Mitchell L. Decalcification during orthodontic treatment with fixed appliance — an overview. Br J Orthod 1992;19(3):199-205.

Shrestha S, Shrestha RM, Prevalence of white spot lesion in nepalese patients with fixed orthodontic appliance. Orthodontic Journal of Nepal.2013;3(2):7-10 DOI:

Life Technologies. Real-time PCR handbook. Available From:

Penders J, Vink C, Driessen C, London N, Thijs C, Stobberingh EE. Quantification of Bifidobacterium spp., Escherichia coli and Clostridium difficile in faecal samples of breast-fed and formula-fed infants by real-time PCR. FEMS Microbiol Lett. 2005;234(1):141-7. DOI: 10.1016/j.femsle.2004.11.052

FDA (2002). Code of Federal Regulations, Title 21-Food and drugs (volume 2), Chapter I-Food and Drug Administration, Department of Health and Human Services, Part 101-Food Labeling-Table of Contents, Subpart A-General Provisions, Sec. 101.14 Health claims: General requirements and Subpart E-Specific requirements for Health Claims. US Government Printing Office, revised as of 1 April 2002. [cited15 Oct 2008] Available from:

Taipale T. Administration of Bifidobacterium animalis subsp. lactis BB-12 and xylitol with a novel pacifier in early childhood [thesis on the internet]. [Turku (Finland)]: University of Turku. 2012:9-56. [Cite 2016 Jan 13]. Available from:

Taipale T, Pienihäkkinen K, Salminen S, JokelaJ, Söderling E. Bifidobacterium animalis subsp. lactis BB-12 Administration in early childhood: A randomized clinical trial of effects on oral colonization by mutans streptococci and the probiotic. Caries Res. 2012;46(1):69-77. DOI: 10.1159/000335567

Caglar E, Sandalli N, Twetman S, Cildir SK, Ergeneli S, Selvi S. Consumption of yogurt with Bifidobacterium DN-173 010 and its effect on dental caries risk factors. Acta Odontol Scand. 2005;63(6):317-20.

Caglar E, Cildir SK, Ergeneli S, Sandalli N, Twetman S. Salivary mutans streptococci and lactobacilli levels after ingestion of the probiotic bacterium Lactobacillus reuteri ATCC 55730 by straws or tablets. Acta Odontol Scand. 2006;64(5):314-8. DOI: 10.1080/00016350600801709

Caglar E, Cildir SK, Kuscu OO, Sandalli N, Holgerson PL, Twetman S. Effect of chewing gums containing xylitol or probiotic bacteria on salivary mutans streptococci and lactobacilli. Clin Oral Investig 2007;11(4):425-9. DOI: 10.1007/s00784-007-0129-9

Garrigues C, Johansen E, Pedersen MB. Complete Genome sequence of Bifidobacterium animalis subsp. lactis BB-12, widely consumed probiotic strain. J Bacteriol. 2010;192(9). DOI: 10.1128/JB.00109-10

Cildir SK, Germec D, Sandalli N, Ozdemir FI, Arun T, Twetmen S, Caglar E. Reduction of salivary mutans streptococci in orthodontic patients during daily consumption of yogurt containing probiotic bacteria. Eur J Orthod. 2009 ; 31(4):407-11.DOI: 10.1093/ejo/cjn108

Caglar E, Kuscu OO, Kuvvetli SS, Cildir SK, Sandalli N, Twetman S. Short-term effect of ice-cream containing Bifidobacterium lactis BB-12 on the number of salivary mutans streptococci and lactobacilli. Acta Odontol Scand. 2008;66(3):154-8. DOI: 10.1080/00016350802089467

Stamatova I, Meurman JH. Probiotics: Health benefits in the mouth. Am J Dent. 2009; 22(6):329-38

Becker MR, Paster BJ, Leys EJ, Moeschberger ML, Kenyon SG, Galvin JL, et al. Molecular analysis of bacterial species associated with childhood caries. J Clin Microbiol.2002;40(3):1001-9

Reddy RS, Swapna LA, Ramesh T, Singh TR, Vijayalaxmi N, Lavanya R. Bacteria in oral health – probiotics and prebiotics: a review. Int J Biol Med Res. 2011; 2(4): 1226-33

Chaturvedi S, Jain U. Importance of probiotics in orthodontics. J Orofac Res. 2015;5(3): 99-103. DOI: 10.5005/jp-journals-10026-1190



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